{"id":3712,"date":"2020-05-04T15:49:26","date_gmt":"2020-05-04T14:49:26","guid":{"rendered":"http:\/\/potravinyx.cz\/?p=3712"},"modified":"2020-05-04T15:50:34","modified_gmt":"2020-05-04T14:50:34","slug":"pomerancovniky-odolne-vuci-virovym-chorobam","status":"publish","type":"post","link":"https:\/\/potravinyx.cz\/?p=3712","title":{"rendered":"Pomeran\u010dovn\u00edky odoln\u00e9 v\u016f\u010di virov\u00fdm chorob\u00e1m"},"content":{"rendered":"\n

T\u00fdm v\u011bdc\u016f z kalifornsk\u00e9 univerzity (University of California, Department of Microbiology and Plant Pathology) a argentinsk\u00e9ho biotechnologick\u00e9ho institutu (Instituto de Biotecnolog\u00eda y Biolog\u00eda Molecular) \u00fasp\u011b\u0161n\u011b vyp\u011bstoval pomeran\u010dovn\u00edky odoln\u00e9 v\u016f\u010di virov\u00e9 n\u00e1kaze, a to s pomoc\u00ed kombinace prost\u0159edk\u016f tradi\u010dn\u00ed a modern\u00ed biotechnologie. Hlavn\u00edm c\u00edlem t\u00e9to studie bylo z\u00edskat pomeran\u010dovn\u00edky rezistentn\u00ed v\u016f\u010di citrusov\u00e9 psor\u00f3ze (CPsV). V\u00fdsledky jejich v\u00fdzkumu jsou publikov\u00e1ny v \u010dasopise \u201eTransgenic Research\u201c.<\/strong><\/h2>\n\n\n\n

Jeden z hlavn\u00edch probl\u00e9m\u016f pro p\u011bstitele pomeran\u010dovn\u00edk\u016f p\u0159edstavuje choroba virov\u00e9ho p\u016fvodu –  citrusov\u00e1 psor\u00f3za (CPsV). Mezi jej\u00ed p\u0159\u00edznaky pat\u0159\u00ed nap\u0159. \u0161upinatost k\u016fry, skvrnitost list\u016f a pomeran\u010d\u016f a \u010dasto i nekr\u00f3za mlad\u00fdch letorost\u016f. Pomeran\u010dovn\u00edky nemaj\u00ed p\u0159irozenou rezistenci v\u016f\u010di tomuto viru, co\u017e se v\u011bdci rozhodli zm\u011bnit prost\u0159ednictv\u00edm (mezi)roubov\u00e1n\u00ed \u2013 vlo\u017een\u00ed geneticky modifikovan\u00e9ho (GM) pletiva mezi netransgenn\u00ed podno\u017e a plodnou odr\u016fdu.<\/p>\n\n\n\n

Na klasickou podno\u017e byla naroubov\u00e1na mal\u00e1 \u010d\u00e1st linie CPsV-rezistentn\u00edho transgenn\u00edho pomeran\u010dovn\u00edku Sweet Orange, a na ni naroubov\u00e1n \u0161t\u011bp netransgenn\u00edho pomeran\u010dovn\u00edku t\u00e9\u017ee odr\u016fdy Sweet Orange. Tato vmeze\u0159en\u00e1 transgenn\u00ed linie exprimuje intronovou sekvenci (ihp) RNA odvozenou z virov\u00e9ho proteinov\u00e9ho obalu (ihpCP).<\/p>\n\n\n\n

V\u00fdsledkem bylo, \u017ee z meziroubovan\u00e9 ihpCP linie p\u0159ech\u00e1zely transkripty (p\u0159episy genetick\u00e9 informace z DNA do RNA) na ne-GM \u0161t\u011bp, a tak se poda\u0159ilo i na netransgenn\u00ed \u010d\u00e1sti stromu inhibovat c\u00edlov\u00e9 m\u00edsto ve virov\u00e9m proteinov\u00e9m obalu.<\/p>\n\n\n\n

Dal\u0161\u00edmi anal\u00fdzami bylo prok\u00e1z\u00e1no, \u017ee exprese ihpCP v transgenn\u00ed \u010d\u00e1sti roubovan\u00e9ho pomeran\u010dovn\u00edku poskytovala cel\u00e9 svrchn\u00ed ne-GM \u010d\u00e1sti stromu odolnost v\u016f\u010di citrusov\u00e9 psor\u00f3ze (i kdy\u017e odolnost v\u00fdsledn\u00e9ho ne-GM \u0161t\u011bpu z \u010d\u00e1sti z\u00e1le\u017eela na m\u00edst\u011b vniknut\u00ed viru).<\/p>\n\n\n\n

V\u00fdsledky studie nazna\u010duj\u00ed, \u017ee meziroubov\u00e1n\u00ed je slibnou biotechnologickou alternativou k ochran\u011b d\u0159evin p\u0159ed virov\u00fdmi infekcemi u vegetativn\u011b se rozmno\u017euj\u00edc\u00edch rostlin.<\/p>\n\n\n\n

Pak u\u017e z\u00e1le\u017e\u00ed jen na pot\u0159ebn\u00e9 velikosti vmeze\u0159en\u00e9ho pletiva k zaji\u0161t\u011bn\u00ed dostate\u010dn\u00e9 produkce transkript\u016f pro ochranu cel\u00e9ho stromu.<\/p>\n\n\n\n

Obdobn\u011b by bylo mo\u017en\u00e9 nap\u0159. v \u010cR pou\u017e\u00edt GM \u0161vestku dom\u00e1c\u00ed, p\u011bstovanou v pokusn\u00e9m re\u017eimu od roku 2001 V\u00fdzkumn\u00fdm \u00fastavem rostlinn\u00e9 v\u00fdroby v Ruzyni, v.v.i. pro podobnou \u00fa\u010dinnost proti viru \u0161arky \u0161vestky.<\/p>\n\n\n\n

Zdroje:<\/strong>
https:\/\/link.springer.com\/article\/10.1007\/s11248-020-00191-1<\/a>,
http:\/\/www.isaaa.org\/kc\/cropbiotechupdate\/article\/default.asp?ID=17950<\/a>, https:\/\/www.vurv.cz\/sites\/File\/Publications\/ISBN978-80-7427-032-1.pdf<\/a>.<\/p>\n\n\n\n

Zdroj \u010dl\u00e1nku: BIOTRIN<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

T\u00fdm v\u011bdc\u016f z kalifornsk\u00e9 univerzity (University of California, Department of Microbiology and Plant Pathology) a argentinsk\u00e9ho biotechnologick\u00e9ho institutu (Instituto de Biotecnolog\u00eda y Biolog\u00eda Molecular) \u00fasp\u011b\u0161n\u011b vyp\u011bstoval pomeran\u010dovn\u00edky odoln\u00e9 v\u016f\u010di virov\u00e9 n\u00e1kaze, a to s pomoc\u00ed kombinace prost\u0159edk\u016f [v\u00edc]<\/a><\/p>\n<\/div>","protected":false},"author":1,"featured_media":3713,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[17],"tags":[396,395,397],"class_list":["post-3712","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-onemocneni","tag-citrusova-psoroza","tag-pomerancovnik","tag-viry"],"views":2091,"_links":{"self":[{"href":"https:\/\/potravinyx.cz\/index.php?rest_route=\/wp\/v2\/posts\/3712","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/potravinyx.cz\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/potravinyx.cz\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/potravinyx.cz\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/potravinyx.cz\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3712"}],"version-history":[{"count":0,"href":"https:\/\/potravinyx.cz\/index.php?rest_route=\/wp\/v2\/posts\/3712\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/potravinyx.cz\/index.php?rest_route=\/wp\/v2\/media\/3713"}],"wp:attachment":[{"href":"https:\/\/potravinyx.cz\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3712"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/potravinyx.cz\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3712"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/potravinyx.cz\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3712"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}